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Intended use
The G-6-PDH Deficiency Screen by Spot Test is a qualitative test is intended for the qualitative, visual fluorescence screening of G-6-PDH in whole blood. Samples which have been determined deficient or intermediate should be assayed by a quantitative G-6-PDH method.
Kit specifications
Trizma Buffer Solution – 1 x 12 ml
G-6-PDH Substrate – 5 x 2 ml
Principle
The G-6-PDH Screening test is a qualitative test performed by incubating a small amount of blood with glucose-6-phosphate and nicotinamide adenine dinucleotide phosphate (NADP). Drops of the mixture are removed at 5-minute intervals, spotted on filter paper and then viewed under long-wave ultraviolet light. Fluorescence is clearly evident in mixtures prepared from normal blood, whereas deficient samples yield little or no fluorescence.